Asayansi apanga enzyme yomwe imatha kukulitsa kuchuluka kwa kuwonongeka kwa pulasitiki kasanu ndi kamodzi. Enzyme yomwe imapezeka m'mabakiteriya a zinyalala omwe amadya zakudya zamabotolo apulasitiki agwiritsidwa ntchito limodzi ndi PETase kuti ipangitse kuwonongeka kwa pulasitiki.
Katatu ntchito ya enzyme wapamwamba
Gululi linapanga enzyme yachilengedwe ya PETase mu labotale, yomwe imatha kufulumizitsa kuwonongeka kwa PET pafupifupi 20%. Tsopano, gulu lomwelo la transatlantic laphatikiza PETase ndi "mnzake" (enzyme yachiwiri yotchedwa MHETase) kuti apange kusintha kwakukulu: kungosakaniza PETase ndi MHETase kumatha kukulitsa kuchuluka kwa kuwonongeka kwa PET Kuwirikiza, ndikupanga kulumikizana pakati pa michere iwiri kuti apange "michere yayikulu" yomwe imathandizira izi.
Gululi limatsogozedwa ndi wasayansi yemwe adapanga PETase, Pulofesa John McGeehan, director of the Center for Enzyme Innovation (CEI) ku University of Portsmouth, ndi Dr. Gregg Beckham, wofufuza wamkulu ku National Renewable Energy Laboratory (NREL). Ku U.S.
Pulofesa McKeehan adati: Ine ndi Greg tikukamba za momwe PETase imawonongera mawonekedwe apulasitiki, ndipo MHETase amapasulanso, chifukwa chake ndichachilengedwe kuwona ngati tingazigwiritse ntchito limodzi kutsanzira zomwe zimachitika m'chilengedwe. "
Mavitamini awiri amagwirira ntchito limodzi
Kuyesera koyambirira kunawonetsa kuti ma enzyme awa amatha kugwira ntchito bwino limodzi, chifukwa chake ochita kafukufuku adaganiza zoyesera kuwalumikiza, monga kulumikiza Pac-Man ndi chingwe.
"Ntchito zambiri zachitika mbali zonse za Atlantic, koma tiyenera kuchita khama - tili okondwa kuwona kuti enzyme yathu yatsopano ya chimeric imathamanga katatu kuposa puloteni yodziyimira payokha yomwe yasintha mwachilengedwe, kutsegulira njira zatsopano zopititsira patsogolo chitukuko ndi kusintha. " McGeehan anapitiliza.
onse awiri a PETase ndi MHETase-PETase omwe angophatikizidwa atha kugwira ntchito pozama pulasitiki ya PET ndikuyibwezeretsanso momwe idapangidwira. Mwanjira imeneyi, mapulasitiki amatha kupangidwa ndikugwiritsidwanso ntchito kosatha, potero kumachepetsa kudalira kwathu pazinthu zakufa monga mafuta ndi gasi.
Pulofesa McKeehan adagwiritsa ntchito synchrotron ku Oxfordshire, yomwe imagwiritsa ntchito ma X-ray, omwe ali olimba kuwirikiza nthawi 10 biliyoni kuposa dzuwa, ngati maikulosikopu, okwanira kuwona ma atomu amodzi. Izi zidalola gulu lofufuzira kuti lithe kukonza mapangidwe a 3D a MHETase enzyme, potero amawapatsa dongosolo la mamolekyule kuti ayambe kupanga makina a enzyme mwachangu.
Kafukufuku watsopanoyu akuphatikiza njira zomangamanga, zowerengera, zama biochemical ndi bioinformatics kuwulula kumvetsetsa kwamamolekyu momwe amagwirira ntchito. Kafukufukuyu ndi gulu lalikulu logwira ntchito asayansi m'magawo onse pantchito.
