Oososayensi benze i-enzyme enokunyusa izinga lokubola kweplastiki amaxesha amathandathu. I-enzyme efunyenwe kwindlu yenkunkuma eyondla ukutya kwiibhotile zeplastikhi isetyenzisiwe ngokudibanisa nePETase ukukhawulezisa ukubola kweplastiki.
Amaxesha amathathu umsebenzi we-enzyme ephezulu
Iqela liyile i-enzyme yendalo ye-PETase elebhu, enokukhawulezisa ukubola kwe-PET malunga ne-20%. Ngoku, iqela elinye le-transatlantic lidibanise i-PETase kunye "neqabane" layo (i-enzyme yesibini ebizwa ngokuba yi-MHETase) ukuvelisa ukuphucula nangakumbi: ngokuxuba nje i-PETase kunye neMHETase kunokunyusa izinga lokuchithwa kwe-PET Ukuphindwe kabini, kunye noyilo lonxibelelwano phakathi kwee-enzymes ezimbini ukwenza "i-enzyme ephezulu" ewuphinda kathathu lo msebenzi.
Iqela likhokelwa sisazinzulu esiyile i-PETase, uNjingalwazi John McGeehan, umlawuli weZiko le-Enzyme Innovation (i-CEI) kwiDyunivesithi yasePortsmouth, kunye noGqirha Gregg Beckham, umphandi omkhulu kwiLebhu yaMandla aVuselelekayo kaZwelonke (NREL). E-U.S.
UNjingalwazi McKeehan uthe: Mna noGreg sithetha ngendlela iPETase ewonakalisa ngayo umphezulu weplastiki, kwaye iMHETase iyaqhubeka nokuwutshabalalisa, ke kuyindalo ukubona ukuba singazisebenzisa kunye ukulinganisa okwenzeka kwindalo. "
Ii-enzymes ezimbini zisebenza kunye
Uvavanyo lokuqala lubonise ukuba ezi enzymes zinokusebenza ngcono kunye, ke abaphandi bagqibe kwelokuba bazame ukuzidibanisa ngokomzimba, njengokudibanisa iPac-Man ezimbini ngentambo.
Umsebenzi omninzi wenziwe kumacala omabini eAtlantic, kodwa kufanelekile ngomzamo-siyavuya ukubona ukuba i-enzyme yethu entsha iyakhawuleza ngokuphindwe kathathu kune-enzyme ezimeleyo yendalo, ivula iindlela ezintsha zophuhliso. kunye nophuculo. " Waqhubeka uMcGeehan.
Zombini i-PETase kunye ne-MHETase-PETase esandula ukudityaniswa inokusebenza ngokwetyisa iplastiki ye-PET kwaye iyibuyisele kulwakhiwo lwayo lwantlandlolo. Ngale ndlela, iiplastiki zinokwenziwa kwaye ziphinde zisetyenziswe ngokungapheliyo, ngaloo ndlela kuncitshiswa ukuxhomekeka kwethu kwizibonelelo zamandulo ezifana neoyile kunye negesi yendalo.
UNjingalwazi McKeehan usebenzise isynchrotron eOxfordshire, esebenzisa ii-X-ray, eziphindwe kalishumi kuneebhiliyoni kunelanga, njengemakroskopu, ngokwaneleyo ukubona iiathom ezizodwa. Oku kuvumele iqela lophando ukuba lisombulule ubume be-3D ye-MHETase enzyme, ngaloo ndlela ibabonelela ngemodeli yeemolekyuli ukuba baqale ukuyila iinkqubo ze-enzyme ezikhawulezayo.
Olu phando lutsha ludibanisa indlela yolwakhiwo, ikhompyuter, iindlela zebhayoloji kunye ne-bioinformatics ukuveza ukuqonda kwemolekyuli kubume bayo kunye nokusebenza. Olu phando ngumzamo omkhulu weqela elibandakanya izazinzulu kuwo onke amabakala emisebenzi.
