Ososayensi benze i-enzyme engakhuphula izinga lokubola kwepulasitiki izikhathi eziyisithupha. I-enzyme etholakala kumagciwane endlu kadoti aphila ngokudla kwamabhodlela epulasitiki isetshenzisiwe ngokuhlangana nePETase ukusheshisa ukubola kwepulasitiki.
Kathathu umsebenzi we-enzyme enkulu
Ithimba lenze i-enzyme yemvelo ye-PETase elabhoratri, engasheshisa ukubola kwe-PET cishe ngama-20%. Manje, iqembu elifanayo le-transatlantic lihlanganise i-PETase kanye "nomlingani" wayo (i-enzyme yesibili ebizwa nge-MHETase) ukukhiqiza ukuthuthuka okukhulu kakhulu: ukumane uxube i-PETase ne-MHETase kungakhuphula izinga lokubola kwe-PET Kukuphindaphinde, futhi kudale ukuxhumana phakathi kwama-enzyme ukudala "i-enzyme enhle" ephinda kathathu lo msebenzi.
Leli thimba liholwa ngusosayensi owaklama iPETase, uSolwazi John McGeehan, umqondisi weCentre for Enzyme Innovation (CEI) e-University of Portsmouth, noDkt Gregg Beckham, ongumcwaningi omkhulu eNational Renewable Energy Laboratory (NREL). E-U.S.
USolwazi McKeehan uthe: Mina noGreg sikhuluma ngokuthi iPETase igugula kanjani ingaphezulu lepulasitiki, kanti uMHETase uyaqhubeka nokuyisakaza, ngakho-ke kungokwemvelo ukubona ukuthi singakusebenzisa yini ndawonye ukulingisa okwenzeka emvelweni. "
Ama-enzyme amabili asebenza ndawonye
Ukuhlolwa kokuqala kukhombisile ukuthi la ma-enzyme angasebenza kangcono ngokubambisana, ngakho-ke abacwaningi banquma ukuzama ukuwaxhuma ngokomzimba, njengokuxhuma iPac-Man ezimbili ngentambo.
"Umsebenzi omningi wenziwe ezinhlangothini zombili ze-Atlantic, kodwa kuwufanele umzamo-siyajabula ukubona ukuthi i-enzyme yethu entsha ishesha ngokuphindwe kathathu kune-enzyme ezimele eguquke ngokwemvelo, kuvula izindlela ezintsha zokuqhubeka nokwenza ngcono. " UMcGeehan uqhubeke.
Kokubili i-PETase kanye ne-MHETase-PETase esanda kuhlanganiswa ingasebenza ngokugaya i-PET plastic iphinde iyibuyisele esimweni sayo sokuqala. Ngale ndlela, amapulasitiki angenziwa futhi asetshenziswe kabusha ngokungapheli, ngaleyo ndlela anciphise ukuncika kwethu emithonjeni yezinsalela ezifana no-oyela negesi yemvelo.
UProfessor McKeehan wasebenzisa i-synchrotron e-Oxfordshire, esebenzisa ama-X-ray, aqine ngokuphindwe izikhathi eziyizigidi eziyizinkulungwane eziyishumi kunelanga, njenge-microscope, anele ukubuka ama-athomu ngamanye. Lokhu kuvumele ithimba locwaningo ukuthi lixazulule ukwakheka kwe-3D kwe-enzyme ye-MHETase, ngaleyo ndlela ibanikeze uhlelo lwamangqamuzana ukuze baqale ukuklama amasistimu enzyme asheshayo.
Lolu cwaningo olusha luhlanganisa izindlela zokwakha, ezezamakhompiyutha, ezamakhemikhali ezemvelo neze-bioinformatics ukuveza ukuqonda kwamangqamuzana ngesakhiwo nokusebenza kwawo. Lolu cwaningo lungumzamo omkhulu weqembu obandakanya ososayensi bazo zonke izigaba zomsebenzi.
